E17-06: Two-Dimensional Liquid Chromatography for Pharmaceutical Analysis

One-Day Course
Sunday, November 12; 8:30am – 5:00pm
  Dr. Dwight Stoll, Gustavus Adolphus College, St. Peter, MN

In this course we will provide a survey of important theoretical and practical issues to consider when developing two-dimensional liquid chromatography methods for pharmaceutical analysis. We will begin with a discussion of the potential advantages of 2D methods over 1D-LC separations, and show examples of 2D methods for solving real analytical problems in pharmaceutical analysis. We will discuss heartcutting, comprehensive, and hybrid modes of 2D separation, and both small and large molecule analyses. We will provide an overview of current understanding of factors that affect 2D separation performance, including the impacts of undersampling, orthogonality of separation mechanisms, and detection sensitivity. Finally, we will discuss current approaches to quantitation and visualization of 2D data, and review the characteristics of commercially available instrumentation for 2D separations.

This one-day course is for is people who are either considering implementing two-dimensional separations for pharmaceutical analysis, or have already purchased and instrument are seeking to quickly learn the fundamentals of the technique and best practices for method development to make the most of their effort with the approach.

1. Theoretical Advantages of 2D-LC Over 1D-LC 
     * Overview of heartcutting, comprehensive, and hybrid 2D separations
     * Review of advantages of 2D for small and large molecule separations
2. Theoretical Considerations for Implementation of 2D-LC 
     * Impact of first dimension undersampling on 2D separation performance 
     * Separation orthogonality and separation mechanisms 
     * Impact of method development decisions on detection sensitivity 
     * Optimization of performance
3. Practical Factors for Implementation of 2D-LC 
     * Compatibility of first and second dimension separations 
     * Optimization of instrument configuration for low level detection 
     * Influence of pumping system design and operation on 2D separation speed and performance
4. Applications 
     * Small molecule 
     * Large molecule 
5. Data Analysis in 2D-LC  
     * Approaches to quantitation and visualization of 2D chromatograms 
     * Overview of advanced chemometric treatment of 2D data 
6. Survey of Commercially Available Instruments and Components for 2D-LC
7. Questions & Answers

Dr. Dwight Stoll did his undergraduate work at Minnesota State University, Mankato, receiving B.S. degrees in plant biology and biochemistry in 1999 and 2001.  Upon graduation in 1999 he took a job in industry as a research technician with ZirChrom Separations, Inc.  At ZirChrom he quickly learned about the liquid chromatography market, and in fact became quite interested in the role of separation science in the development of new analytical methodologies for use in other disciplines such as biology.  In 2000 he shored up his chemistry background at the University of Minnesota before enrolling in the graduate program in chemistry there in 2001.  At the University of Minnesota he studied with Professor Peter Carr, and worked on the development of Fast, Comprehensive Two-Dimensional Liquid Chromatography, using the principles of high temperature and ultra-fast gradient elution liquid chromatography to improve the overall speed of two-dimensional separations.  Before receiving the Ph.D. in analytical chemistry in 2007, he took a nine-month break from graduate studies to teach as an adjunct faculty member at St. Olaf College where he taught analytical and general chemistry.  Following graduate in 2007, he spent nine months working as a post-doctoral researcher with Dr. Christine Wendt in the Lung Health Center at the University of Minnesota, where he began analyzing the low molecular weight constituents of human lung lavage fluid using liquid chromatography coupled with mass spectrometry.

In the fall of 2008, Dwight accepted a faculty position as Assistant Professor in the Chemistry Department at Gustavus Adolphus College, where he mainly teaches quantitative and instrumental analysis courses, in addition to directing a vibrant research program involving mainly undergraduate students.  In 2014 he was promoted to Associate Professor at Gustavus, and is currently co-chair of the Chemistry Department. His active research projects include the development of rapid multidimensional liquid chromatography for both targeted and untargeted analysis in samples of moderate to high complexity. Active research projects in his laboratory touch upon most aspects of multidimensional separation methodologies, including optimization of isocratic and gradient elution HPLC, characterization of selectivity in reversed-phase HPLC, instrument development, and applications in biopharmaceutical analysis.

Dwight is the author or co-author of 49 peer-reviewed publications and two book chapters in the area of separation science, and is a named co-inventor on four patents.  He has authored or co-authored over 95 presentations at local, national, and international meetings, and has instructed numerous short courses in two-dimensional liquid chromatography.  In 2009 he was the winner of the John B. Phillips Award for contributions to multidimensional gas chromatography, and in 2011 he was the recipient of LCGC’s Emerging Leader in Chromatography Award. In 2012 he was elected to the editorial advisory board of LCGC Magazine, the leading trade publication for the separation science community. In 2014 he was named to The Analytical Scientist’s list of ‘Top 40 Under 40’ analytical scientists, and in 2015 he received the American Chemical Society Division of Analytical Chemistry Award for Young Investigators in Separation Science. In 2016 he received the Palmer Award from the Minnesota Chromatography Forum, and the Gustavus Faculty Scholarly Achievement Award. In 2017 he will receive the Eastern Analytical Young Investigator Award, and the Georges Guiochon Faculty Fellowship.