E18: Supercritical Fluid Chromatography (SFC): A Powerful and Greener Tool for Analytical and Preparative Separations

One-Day Course 
Date is TBA; 8:30am – 5:00pm

Dr. Yingru Zhang, Bristol-Myers Squibb, Princeton, NJ
Dr. Michael Hicks, Merck & Co., Inc, Rahway, NJ

SFC is becoming a mainstream technique in recent years for its complementarity to LC and GC with orthogonal selectivity, enhanced speed and efficiency, and lower solvent usage, thus “greener”. This course provides an introduction to SFC fundamentals and method development with practical instructions on implementing SFC as an everyday separation technique to complement other chromatography. First, we cover practical knowledge necessary to develop successful SFC methods taking participants step by step through the instrument setup and configurations, column and mobile phase selections and SFC conditions for both chiral and achiral applications. Second, we will guide the participants through physical properties and chromatography attributes to understand the power of SFC. Finally, we cover the intrinsic advantages of modern SFC technology and its expansion and strategies for more experienced liquid chromatographers to develop a broader spectrum of SFC applications.

This course is open to any analysts and chromatographers looking to enhance their knowledge and skillset to include SFC. Current SFC users looking to develop or advance their method development knowledge will find this course helpful for analytical and preparative applications.

1. Introduction to Supercritical Fluid Chromatography
a. Instrumentation setup and configurations
b. Similarity and difference from GC and HPLC
2. SFC method development strategy
a. Stationary phase selection – from recent advances and systemic studies
b. Role of modifiers and additives in packed column SFC – the saver of SFC
c. Temperature and pressure – a new dimension of power and flexibility
d. Detection – UV, CD, ELSD, CAD, MS and FID
3. SFC Physical Attributes and Practical Applications
a. Enantioselective separation for problem solving-analytical challenges (SFC/UV/CD)
b. Achiral applications-orthogonal selectivity
c. Preparative SFC and simulated moving bed (SMB)-SFC
d. Bioanalysis using SFC/MS/MS
4. The future of SFC – bridging the gaps for unity in separation science
a. Newer applications of SFC in the Pharmaceutical Industry
     b. SFC for GMP applications from drug substance to drug product
     c. Popular misconceptions about SFC
     d. Current issues and barriers to consider when contracting out methods

Dr. Yingru Zhang is a Senior Principal Scientist at Bristol-Myer Squibb Company (BMS) leading Separation and Physical Science groups supporting Drug Discovery from small to bio-molecules in NJ. He obtained his Ph.D. in Physical Chemistry under Prof. Aronson at City University of New York. Since then, he has had 28 years research and development experience at American Cyanamid, BASF, Wyeth and BMS in both the discovery and development arena. He has extensive experience in SFC, and established and managed the Separation Groups with leading edge SFC capabilities at Wyeth and BMS. He was involved in early adoption and development of Berger SFCs (MG-I,II,III). Dr. Zhang pioneered Simulated Moving Column (SMC) technique for SFC, and developed Multi-Column Parallel Screening with Circular Dichroism Signal Pooling for rapid method development. He has over 30 publications in peer reviewed journals including recent SFC papers in Analytical Chemsitry and JChrom A, as wel as coauthered book chapters on SFC.

Dr. Michael Hicks has over 20 years of pharmaceutical research experience with a diversified background ranging from early discovery to pharmaceutical pre-clinical R&D. Dr. Mike started in Analytical Research and Development in 1989 at Merck, received his PhD from Seton Hall University in 1997. He taught an Advanced Biomedical Engineering Graduate Laboratory at the Stevens Institute of Technology as adjunct faculty on Saturdays for ten years. He currently serves on the Board of Directors for the Eastern Analytical Symposium. He has been working in Separations Sciences area for over 15 years and is currently in the Analytical Chemistry and Enabling Technologies group in the Analytical Research & Development Department in Merck Research Labs at Merck & Co. Inc. in Rahway as an SFC subject matter expert.