E19: Modern Size Exclusion Chromatography of Synthetic Polymers and Biopolymers

One-Day Course 
Date to be announced; 8:30am – 5:00pm

Dr. Howard G. Barth, Analytical Chemistry Consultants, Ltd, Wilmington, DE
Dr. Brian A. Bidlingmeyer,  Analytical Acumen, LLC, Wilmington, DE

This one-day course covers the fundamentals and practice of size exclusion chromatography (SEC) of synthetic polymers and biopolymers with emphasis on the separation mechanism, average molecular weights and distribution, column calibration approaches, SEC methods development of organosoluble and water-soluble polymers, detection systems including viscometry and light scattering, and applications. Attendees will have an opportunity to discuss specific problems related to polymer characterization in general or SEC methods development or column calibration.

This course is geared for chemists or technicians with little or no experience in size exclusion chromatography, or for those familiar with the technique but desire a more thorough understanding of SEC fundamentals and applications.


  1. Macromolecules and their Properties
  2. Overview and Definitions of SEC
  3. Applications of SEC
  4. SEC of Small Molecules
  5. Separation Mechanism
  6. Peak Broadening
  7. Thermodynamics of SEC
  8. Relationship between SEC and LC
  9. Average MWs and MWD
  10. Column Calibration
  11. Operating Conditions
  12. Sample Preparation
  13. Column and Packing Selection
  14. Mobile Phase Selection
  15. Concentration Detectors
  16. Light Scattering and Viscometric Detectors
  17. Accuracy/Reproducibility
  18. Future Directions

Dr. Howard G. Barth is a noted veteran chromatographer, received a PhD in analytical chemistry from Northeastern University (1973). After a postdoctoral fellowship, he began his career at Hercules Research Center, then joined the DuPont Company, where he retired in 2007.

Howard is cofounder of the International Symposium on Polymer Analysis and Characterization, which he chaired for many years. He was appointed assistant editor of the Journal of Applied Polymer Chemistry, and served as series editor of Springer Lab Manuals in Polymer Science. Howard is founder and was editor-in-chief of the International Journal of Polymer Analysis and Characterization. He has authored or coauthored about 110 technical articles, including book chapters, edited or co-edited books, and a coauthored text on size exclusion chromatography. At present he writes a bimonthly column on Chromatography Fundamentals for LCGC North America. He consults in the areas of polymer characterization, separation science, and general analytical chemistry.

Dr. Brian A. Bidlingmeyer is is an accomplished scientist in the field of separation science and has published over 100 technical papers and authored two books, one on practical HPLC and the other on preparative chromatography.  Brian has a wide range of work experience including the pharmaceutical, the industrial chemical and the instrumentation industries.  He has worked for Amoco Chemicals as a research chemist, for Waters as Vice President/Technical Director, for Sterling Winthrop Pharmaceuticals as an Assistant Director.  He was a co-founder of Cohesive Technologies and was employed by Agilent Technologies where he developed new column technologies and applications.  After retiring from Agilent, he formed Analytical Acumen, LLC, a consulting and technology company.  Brian has received numerous awards and it is noteworthy that Brian is among the few who have been selected as a Fellow of the American Chemical Society.