E19-16: Modern Size Exclusion Chromatography of Synthetic Polymers and Biopolymers

One-Day Course 
Sunday, Nov. 17; 8:30am – 5:00pm

Dr. Howard G. Barth, Analytical Chemistry Consultants, Ltd, Wilmington, DE
Dr. Brian A. Bidlingmeyer,  Analytical Acumen, LLC, Wilmington, DE

This comprehensive, one-day course on size exclusion chromatography (SEC) will provide details on developing and evaluating SEC methods for a range of polymeric materials and biopolymers. The principles of SEC, including the mechanism of separation and the significance of average molecular weights will be given. Emphasis is placed on column calibration, and the selection and evaluation of columns and mobile phases. Fundamental principles of light scattering and viscometry will be fully explained, including branching and molecular conformation measurements. Attendees will have an opportunity to discuss specific problems related to polymer characterization or SEC methods development and column calibration.

This course is geared for chemists or technicians with little or no experience in size exclusion chromatography, or for those familiar with the technique but desire a more thorough understanding of SEC fundamentals and applications.


  1. Introduction to SEC
  2. Overview of Applications
  3. MWD/MW Averages
  4. Characteristics of Synthetic/Biopolymers
  5. Fundamentals of SEC
  6. Column Calibration Approaches
  7. Theoretical Plates
  8. Peak Broadening
  9. Column Resolution
  10. Practice of SEC
  11. Column Selection
  12. Mobile Phase Selection
  13. SEC Method Validation
  14. Accuracy/Reproducibility
  15. Limitations of SEC
  16. Viscometry
  17. Light Scattering
  18. Compositional Heterogeneity
  19. SEC State-of-the-Art
  20. Open Discussion

Dr. Howard G. Barth is a noted veteran chromatographer, received a PhD in analytical chemistry from Northeastern University (1973). After a postdoctoral fellowship, he began his career at Hercules Research Center, then joined the DuPont Company, where he retired in 2007.

Howard is cofounder of the International Symposium on Polymer Analysis and Characterization, which he chaired for many years. He was appointed assistant editor of the Journal of Applied Polymer Chemistry, and served as series editor of Springer Lab Manuals in Polymer Science. Howard is founder and was editor-in-chief of the International Journal of Polymer Analysis and Characterization. He has authored or coauthored about 110 technical articles, including book chapters, edited or co-edited books, and a coauthored text on size exclusion chromatography. At present he writes a bimonthly column on Chromatography Fundamentals for LCGC North America. He consults in the areas of polymer characterization, separation science, and general analytical chemistry.

Dr. Brian A. Bidlingmeyer is is an accomplished scientist in the field of separation science and has published over 100 technical papers and authored two books, one on practical HPLC and the other on preparative chromatography.  Brian has a wide range of work experience including the pharmaceutical, the industrial chemical and the instrumentation industries.  He has worked for Amoco Chemicals as a research chemist, for Waters as Vice President/Technical Director, for Sterling Winthrop Pharmaceuticals as an Assistant Director.  He was a co-founder of Cohesive Technologies and was employed by Agilent Technologies where he developed new column technologies and applications.  After retiring from Agilent, he formed Analytical Acumen, LLC, a consulting and technology company.  Brian has received numerous awards and it is noteworthy that Brian is among the few who have been selected as a Fellow of the American Chemical Society.