E19: High-Performance TLC, Planar Chromatography Beyond the Ordinary!

One-Day Course 
Date to be announced; 8:30am – 5:00pm

Dr. Eike Reich, The International Association for the Advancement of High Performance Thin Layer Chromatography, Rheinfelden, Switzerland
Dr. Maged Sharaf, The International Association for the Advancement of High Performance Thin Layer Chromatography, Rheinfelden, Switzerland

Unlike conventional TLC, high-performance thin-layer chromatography (HPTLC) is a modern planar chromatographic technique with rigorous standardization, sophisticated instrumentation and software, validated methods and documented procedures. It offers higher resolution, uses less solvent and time. HPTLC achieves reproducible results in a cGxP environment, thus allowing direct analytical comparison of multiple samples across time and different laboratories. The unique properties of HPTLC fingerprints can answer various analytical questions and open new possibilities of describing the quality of complex samples. This course will discuss concepts, parameters and unique features of HPTLC, examine case studies demonstrating its versatility, and display its practical aspects.

Researchers, scientists, academicians, QA/QC/R&D personnel, lab managers and technicians. Anyone wanting to learn more about HPTLC and how it might benefit your lab.


  1. Concepts, Parameters, and Advances of HPTLC
    a. What is different in HP-TLC?
    b. Standardization – the magic bullet
    c. HPTLC in the pharmacopoeias
    d. HPTLC hyphenations – flexibility for research
    e. HPTLC – the future is here!
  2. Comprehensive HPTLC Fingerprinting
    a. HPTLC – the other high-performance chromatography
    b. The HPTLC fingerprint
    c. Quantitative information obtained from digital images
    d. Comprehensive HPTLC Fingerprinting: a different look at quality
  3. Quantitation and Spectral Evaluation of Substances by HPTLC
    a. Principles of scanning densitometry
    b. Practical considerations
    c. Beyond “gestimation”
  4. Development and Validation of HPTLC Methods
    a. Method development strategies
    b. Validation of method for identification
    c. Validation of quantitative methods
    d. Proper handling of instruments and software: Demonstration

Dr. Eike Reich received his doctorate degree in Natural Products Chemistry in 1989 from Humboldt University East Berlin. From 1990 to 1998 he was Associate Professor of Chemistry at Longwood College, Virginia, USA. In 1998 he moved to Switzerland to join CAMAG as Head of Laboratory. His research focuses on applications of High Performance Thin-Layer Chromatography. Dr. Reich has published numerous papers on the subject and contributed chapters to textbooks and encyclopedias. He is author of the book “HPTLC for the analysis of medicinal plants”.  Dr. Reich is founding member of the International Association for the Advancement of HPTLC. In May of 2018, he was elected President of the HPTLC Association. In this capacity, he is promoting the use of HPTLC and soliciting international collaboration on the HPTLC Atlas for identification of herbal drugs.

Dr. Maged Sharaf earned a Ph.D. and masters in pharmaceutical sciences with emphasis on pharmacognosy, and a bachelor’s in pharmacy and pharmaceutical sciences. He joined CAMAG Scientific (USA) in 2018 as the director of scientific business development. Before CAMAG, Dr. Sharaf was the chief science officer at the American Herbal Products Association where he helped to set quality standards for the herbal product industry. Before AHPA, he was with the United States Pharmacopeial Convention (USP) assuming several responsibilities including its director Foods, Dietary Supplements and Herbal Medicines. During his tenure at USP, he co-launched two of the USP compendia, developed botanical standards for inclusion in the United States Pharmacopeia-National Formulary (USP-NF), and participated in building the USP Dietary Supplement Verification Programs. Before USP, he was associate professor and taught undergraduate and graduate courses in pharmacognosy and pharmaceutical sciences. He has preceding experience conducting bioanalytical assay development and validation, and human bioequivalence studies in support of the pharmaceutical industry; quality control testing of finished dosage forms; and dosage forms manufacturing. Dr. Sharaf is a founding member (2012), member of the Board of Directors, and chair Method Review Committee of the International Association for the Advancement of High Performance Thin Layer Chromatography; member of USP expert committees, scientific advisor to the American Herbal Pharmacopeia, member of the American Society of Pharmacognosy, a council member of the Specialty Committee of Traditional Chinese Medicines–Pharmaceutical Analysis, World Federation of Chinese Medicine Societies, and a member of several other scientific organizations.