E18-01: Practical Gas Chromatography

Two-Day Course
Sunday, Nov. 11 – Monday, Nov. 12; 8:30am – 5:00pm

Dr. Eugene F. Barry, University of Massachusetts Lowell, Lowell, MA
Dr. Thomas Brettell, Cedar Crest College, Allentown, PA

This two-day course presents the fundamentals of gas chromatography with an emphasis on practical applications for users and method developers. Topics to be covered include theoretical considerations, use of computer searches for literature references and methods of analysis, modern instrumentation, including inlet, column, and detector technology, and the applications of these to effective qualitative and quantitative analysis. The theoretical portion of the course will focus on using the fundamental understanding of the chromatographic process (limited number of equations and comparisons to extractions and distillations) to assist in obtaining a desired separation quality and run time. Modern instrumentation including split, splitless, on-column, and programmed-temperature inlets and electronic pressure control will be discussed. The proper selection of capillary columns to solve practical problems will be described, although the utility of modern packed columns will be briefly discussed. An emphasis will also be placed on detectors, including TCD, FID, ECD and GC/MS. Finally all this will be applied to practical problems in qualitative and quantitative analysis.

This course represents a balanced blend of pertinent information and underlying theory for successful practice of gas chromatography. Individuals working in the area of gas chromatography, beginners and those desiring to update their knowledge of the technique will find this course to be meaningful and useful. The instructors of this short course will welcome Questions and Problems pertinent to the subject material covered in the course prior to the meeting. These questions & problems will be answered in a Question & Answer Session at the end of the course.

1. Theory and Basics  
     a. Evolution of chromatography
     b. IUPAC nomenclature
     c. Similarities to extractions & distillations
     d. Theory of gas chromatography-Plate and Rate Theories
     e. Effect of changing conditions on peak separations 
2. Inlets/mobile Phases
     a. Injection modes & mobile phases
     b. Instrumental requirements for packed and capillary columns
     c. Capillary column inlets (split, splitless, on- column, direct injection, electronic pressure control)
     d. Programmed-temperature vaporizer, large volume injections
     e. Packed column inlets /column selection
     f. Classification and selection of stationary liquid  phases and adsorbents
     g. Capacity and analysis time
3. Capillary Column 
     a. Capillary column selection
     b. Chromatographic parameters affecting column performance
     c. Effect of capillary column ID, film thickness, length &  choice of carrier gas on resolution
     d. Capillary column rinsing, rejuvenation, care and maintenance
4. Detectors
     a. Fundamentals of detector responses
     b. Types of detectors
     c. Detectors used for various analysis
5. Computer Assistance in Gas Chromatography
     a. Internet guidance
     b. Software for prediction and optimization of separations
6. Qualitative and Quantitative Analysis
     a. Qualitative analysis
     b. Quantitative analysis methods

*NOTE: This course covers gas chromatography, not the technique of gas chromatography-mass-spectrometry (GC-MS).  Modern Practice of Gas Chromatography; 4th Edition, Robert L. Grob and Eugene F. Barry, Eds.; John Wiley & Sons: New York, 2004. ISBN 0-471-22983-0

Dr. Eugene F. Barry is Professor Emeritus of Chemistry and former Department Head of the Chemistry Department at the University of Massachusetts Lowell. He received his BS in Chemistry from Villanova University (1967) and a Ph.D. in Analytical Chemistry from the University of Rhode Island (1970). He is a recipient of the Most Outstanding Teaching Award at UMass Lowell. During his tenure at the University of Massachusetts Lowell, Dr. Barry has taught a wide variety of courses in Analytical Chemistry, including graduate level courses in chromatography and separation methods, his primary area of research. He and the late Dr. Robert Grob are co-editors of Modern Practice of Gas Chromatography, Fourth Edition, published by John Wiley & Sons (2004) and authors of the book, Columns for Gas Chromatography: Performance and Selection also published in 2007 by Wiley. His current research interests include GC-MS, computer-assisted optimization of separations by capillary GC, high-speed gas chromatography and the determination of organics in challenging matrices, such as cement and concrete, enhanced oil recovery in addition to geological and oceanic sequestration of carbon dioxide. He is author of over 100 Research Publications and several patents.

Dr. Thomas A. Brettell, the former Director of the New Jersey State Police Office of Forensic Sciences, is presently Associate Professor of Chemistry in the Chemical and Physical Sciences Department at Cedar Crest College where he teaches advanced separation and forensic chemistry courses in the undergraduate and graduate forensic science programs. He received his BA in Chemistry from Drew University (1973) and a Ph.D. in Analytical Chemistry from Villanova University (1987). Dr. Brettell is a past Chair of the Criminalistics Section of the American Academy of Forensic Sciences and the past President of the Chromatography Forum of the Delaware Valley. Tom was presented the Chromatography Forum of the Delaware Valley Award in 1997 for service to the Forum and accomplishments in the field of separation science, and has also served on the advisory Board of the Journal of Analytical Chemistry. Dr. Brettell is a Fellow of the American Board of Criminalistics and a Fellow in the American Academy of Forensic Sciences.