E18-23: Troubleshooting Chromatographic Systems

Two-Day Course
Tuesday, Nov. 13-Wednesday, Nov. 14; 8:30am – 5:00pm

Dr. Merlin K.L. Bicking, ACCTA, Inc., St. Paul, MN
Dr. Douglas E. Raynie, South Dakota State University, SD

This two day short course provides guidance on identifying the causes of many chromatography problems, and offers tips for finding solutions and preventing future problems. Basic LC and GC components will be discussed, and helpful hints will be provided on how to avoid certain problems while maximizing the overall analytical efficiency in the laboratory. Students will learn about general troubleshooting strategies, instrument design issues, common symptoms, and common solutions to common symptoms. This seminar provides practical technical information that is not available from any other single source.

Lab operators and analysts who use one or both techniques. Supervisors who want to learn more about laboratory instrument issues.

1.  A General Approach to Troubleshooting
a. Why are we here?
b. How do we get started with troubleshooting?
2.  Minimizing Errors in Peak Integration 
     a. How do chromatographic integrators work
     b. What integration baseline options are available
     c. Understanding the errors and minimizing them
3.  GC Troubleshooting 
     a. Current trends in instrument design
     b. Comprehensive troubleshooting strategies for GC
     c. Matching symptoms with solutions
d. The GC Quiz
4.  LC Troubleshooting
     a. Design-related problems
     b. Linking symptoms and solutions with the LC Troubleshooting Matrix
c. The LC Quiz
5.  Open Discussion – bring your own examples and questions

Dr. Merlin K. L. Bicking (Course Director) is President and Senior Analytical Scientist, ACCTA, Inc. He has extensive analytical chemistry experience in academia, contract research, independent testing laboratories, consulting, and technical training. His professional history includes development of two EPA methods, as well as numerous methods in other regulated and non-regulated industries. His publications and presentations cover a wide range of topics, including liquid chromatography theory, derivatization, method optimization, and the use of experimental design strategies in analytical chemistry. He also develops and presents technical training seminars for analytical laboratory staff.

Dr. Douglas E. Raynie is a Research Assistant Professor in the Department of Chemistry and Biochemistry at South Dakota State University. Prior to joining SDSU, he was employed for eleven years as a Senior Scientist at Procter and Gamble’s Corporate Research Division. He earned his Ph.D. at Brigham Young University under the direction of Dr. Milton L. Lee. His undergraduate degree is from Augustana (South Dakota) College, with majors in chemistry and
biology. Analytical separations research in Dr. Raynie’s laboratory includes high-resolution chromatography (high-temperature LC and SFC), chromatographic sample preparation  (ASE, SFE, SPME, and SPE), chromatography theory, green analytical chemistry, and problem-based learning in analytical chemistry.